Composite
CBDcex(T7)

Part:BBa_K863102:Design

Designed by: Moritz Müller   Group: iGEM12_Bielefeld-Germany   (2012-09-19)

Cellulose binding Domain of C. Fimi Exoglucanase with T7, RBS, GS-Linker (Freiburg-Standard)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 365
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Biobrick was designed to express the cellulose binding domain of Cellulomonas fimi exoglucanase (CBDcex) under the control of a T7-promoter BBa_K525998. 12 additional conserved bases (4 AS) were isolated upstream of the cellulose binding domain and 9 bases downstream of the exoglucanase gene and also a short C-terminal (Glycine, Serine) linker was added. The sequence ends with the AgeI-restriction-site of the Freiburg-Suffix, to make in-frame (Standard 25) assembly possible.

Primers that were used to make this BioBrick:

CBDcex_T7RBS TGAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGAAAGAGGAGAAATAATGGGTCCGGCCGGGTGCCAGGT
CBDcex_2AS-Link_Frei CTGCAGCGGCCGCTACTAGTATTAACCGGTGCTGCCGCCGACCGTGCAGGGCGTGC

Source

Origin of the cellulose binding domain is a cloning-vector form the fermentation group of Bielefeld University.

References